Deamidation of human proteins

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Deamidation of human proteins.

Deamidation of asparaginyl and glutaminyl residues causes time-dependent changes in charge and conformation of peptides and proteins. Quantitative and experimentally verified predictive calculations of the deamidation rates of 1,371 asparaginyl residues in a representative collection of 126 human proteins have been performed. These rates suggest that deamidation is a biologically relevant pheno...

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Nonenzymatic deamidation of asparaginyl and glutaminyl residues in proteins.

Some asparagine and glutamine residues in proteins undergo deamidation to aspartate and glutamate with rates that depend upon the sequence and higher-order structure of the protein. Functional groups within the protein can catalyze this reaction, acting as general acids, bases, or stabilizers of the transition state. Information from specific proteins that deamidate and analysis of protein sequ...

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Peptidoglutaminase Deamidation of Proteins and Protein Hydrolysates for Improved Food

The limited deamidating ability of peptidoglutaminase {PGase) toward intact food proteins (0-6% deamidation) can be significantly enhanced by prior protein hydrolysis and altering protein conformation by such means as moist heat. PGase deamidation increases protein solubility and improves emulsifying and other physical properties under mildly acidic conditions. A batch reactor method was develo...

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Pasteurella multocida toxin activation of heterotrimeric G proteins by deamidation.

Pasteurella multocida toxin is a major virulence factor of Pasteurella multocida, which causes pasteurellosis in men and animals and atrophic rhinitis in rabbits and pigs. The approximately 145 kDa protein toxin stimulates various signal transduction pathways by activating heterotrimeric G proteins of the Galpha(q), Galpha(i), and Galpha(12/13) families by using an as yet unknown mechanism. Her...

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Pasteurella multocida Toxin Activates Various Heterotrimeric G Proteins by Deamidation

Pasteurella multocida produces a 146-kDa protein toxin (Pasteurella multocida toxin, PMT), which stimulates diverse cellular signal transduction pathways by activating heterotrimeric G proteins. PMT deamidates a conserved glutamine residue of the α-subunit of heterotrimeric G proteins that is essential for GTP-hydrolysis, thereby arresting the G protein in the active state. The toxin substrates...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2001

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.221463198